Proper supply of S increases GSH synthesis in the establishment and reduces tiller mortality during the regrowth of Tanzania guinea grass used for Cd phytoextraction

Purpose

Cadmium has caused serious environmental problems due to its phytotoxicity, requiring solutions to reduce its concentration in the environment. Because S can alleviate the phytotoxicity caused by heavy metals, the evaluation of the effects of S supply is a promising approach to address this problem. The aim of this study was to determine the influence of S in reducing the phytotoxicity caused by Cd to Panicum maximum Jacq. cv. Tanzania (guinea grass) and to evaluate the potential of this grass for Cd phytoextraction.

Materials and methods

Tanzania guinea grass was cultivated in a nutrient solution to evaluate the combinations of five rates of S (0.1, 1.0, 1.9, 2.8, and 3.7 mmol L^?1) and five rates of Cd (0.0, 0.5, 1.0, 1.5, and 2.0 mmol L^?1), in a 5² fractional factorial design with six replications. Six plants were grown per pot in two growth periods to evaluate the effect of Cd on the establishment (51 days of age) and on the regrowth (18 days of growth) of the plants. During regrowth, Cd was not supplied in the nutrient solution. At the end of each growth period, growth parameters, concentrations of S and Cd, Cd transport factor, and concentrations of hydrogen peroxide (H₂O₂), malondialdehyde, reduced glutathione, and oxidized glutathione were evaluated.

Results and discussion

On the establishment of Tanzania guinea grass, application of Cd reduced mass production by 39 % due to the increases in the concentration of Cd (30 times compared with the treatment without Cd) and in lipid peroxidation (124 %). During regrowth, the residual effect of Cd resulted in a lower number of tillers (62 %) and leaves (89 %) caused by the increase in concentrations of Cd, H₂O₂ (49 %), and lipid peroxidation, which resulted in a 65 % lower dry mass production. The proper supply of S reduced concentration of Cd and tiller mortality rate due to the residual effect of Cd on the regrowth of the grass. Mass production by the roots was 43 % lower because of Cd, but the transport of Cd from roots to shoots increased. The increase in the concentration of glutathione (GSH) associated with a possible higher activity of antioxidant enzymes alleviated the phytotoxic effects of Cd on the guinea grass.

Conclusions

The supply of S reduces the phytotoxicity caused by Cd to guinea grass, especially during the regrowth of the plant, when the concentration of Cd is higher because of the transport from roots to shoots. Tanzania guinea grass showed to be very promising for phytoextraction of Cd due to its high mass production, even when Cd was largely available in the nutrient solution.

     

Studies on the colonization of axenically grown tomato plants by a GFP-tagged strain of Clavibacter michiganensis subsp. michiganensis

In this study, colonization and disease development of axenically-grown tomato plants by Clavibacter michiganensis subsp. michiganensis (Cmm), the causative agent of bacterial wilt and canker, was investigated. For this, a spontaneous rifampicin resistant strain of Cmm was tagged with a marker that expressed a green fluorescent protein (GFP) in a stable way and which possessed a similar virulence to the parental strain. In vitro plants were drop-inoculated at the stem base and the population dynamics was determined by dilution pour-plating in a selective medium. At 3 h after inoculation, Cmm was already present in low densities in roots, stems and leaves. At 16 dpi, Cmm was found throughout the entire plant in high densities of ca. 10¹⁰ cfu g^?1. Symptoms developed in the in vitro plants typical for Cmm, such as canker, wilting and growth reduction. The presence of Cmm in vascular and parenchymatic tissue of in vitro tomato plants was confirmed by epifluorescence stereo- and confocal laser scanning microscopy. This study showed that in vitro tomato plants can be effectively used for detailed studies on interactions between Cmm and its host, in particular if a GFP-tagged strain of the pathogen is used.     

Thiophanate-methyl sensitivity and fitness in Lasiodiplodia theobromae populations from papaya in Brazil

Stem-end rot, caused by Lasiodiplodia theobromae, is an important postharvest disease of papaya in Brazil. The use of fungicides is one of the main disease management measures. However, there are no data available on the sensitivity of L. theobromae to thiophanate methyl (methyl benzimidazole carbamate), the most common fungicide used in papaya orchards in northeastern Brazil. Thus, the effective concentration that results in 50 % of mycelial growth inhibition (EC₅₀) of 109 isolates, representing five populations of the pathogen was estimated in vitro. Seven components of fitness were measured for the 10 isolates with lower and high values of EC₅₀. Of the 109 isolates, 20.2 % were resistant to the fungicide with EC₅₀ values greater than 300 μg ml^?1, whereas the remaining 79.8 % were sensitive with an average EC₅₀ of 1.87 μg ml^?1. The EC₅₀ values for the resistant isolates were significantly (P?≤?0.05) higher than those for the sensitive isolates. When the fitness components were evaluated, only in relation to the spore production was significant difference among sensitive and resistant isolates, and resistant isolates showed sporulation capacity significantly lower than the S isolates, indicating a fitness cost.     

Effects of bamboo stands on seed rain and seed limitation in a rainforest

Bamboos often negatively affect tree recruitment, survival, and growth, leading to arrested tree regeneration in forested habitats. Studies so far have focused on the effects of bamboos on the performance of seedlings and saplings, but the influence of bamboos on forest dynamics may start very early in the forest regeneration process by altering seed rain patterns. We tested the prediction that the density and composition of the seed rain are altered and seed limitation is higher in stands of Guadua tagoara (B or bamboo stands), a large-sized woody bamboo native from the Brazilian Atlantic Forest, compared to forest patches without bamboos (NB or non-bamboo stands). Forty 1 m² seed traps were set in B and NB stands, and the seed rain was monitored monthly for 1 year. The seed rain was not greatly altered by the presence of bamboos: rarefied seed species richness was higher for B stands, patterns of dominance and density of seeds were similar between stands, and differences in overall composition were slight. Seed limitation, however, was greater at B stands, likely as a resulted of reduced tree density. Despite such reduced density, the presence of trees growing amidst and over the bamboos seems to play a key role in keeping the seeds falling in B stands because they serve as food sources for frugivores or simply as perches for them. The loss of such trees may lead to enhanced seed limitation, contributing ultimately to the self-perpetuating bamboo disturbance cycle.     

Deforestation and fragmentation of Chaco dry forest in NW Argentina (1972–2007)

The sustained increase in the global food demand has favoured the recent expansion of industrial agriculture into neotropical dry forest ecosystems, with resulting changes in their extent and spatial configuration. Based on Landsat satellite images, we analyzed changes in forest cover and landscape configuration over an area of 600 by 100 km located in NW Argentina (Tucumán and Salta provinces) in four periods between 1972 and 2007. The study area, one of the most active deforestation frontiers of South American dry forest, was divided into six relatively homogeneous sectors in terms of land property structure and biophysical characteristics. During the study period 1.4 millions hectares of dry forest were cleared. Deforestation started in the 1970s as a result of technological changes and increasing rainfall; continued (with spatial and temporal fluctuations) during the 1980s and 1990s in association to the sustained global demand of soybean, and was accelerated (to ca. 100,000 ha year⁻¹) between 2001 and 2007 following the global increase in commodity prices, and the national peso devaluation. We described the landscape structure using eight landscape indices, summarized as one synthesis value: the Euclidian distance across the seven dimensions from a theoretical non-fragmented situation. In areas with soil limitations deforestation resulted in relatively stable, highly fragmented landscapes. In contrast, the sites with regional coarser-scale limitations (rainfall), deforestation produced a less fragmented landscape where agriculture concentrates in sites with high rainfall. Sites with no limitations for agriculture tend to a largely deforested landscape with few small and poorly connected forest patches. The land properties size seems to influence some indices of fragmentation, but the synthetic index of fragmentation suggests an overall convergence of fragmentation patterns towards a similar configuration across different biophysical and land tenure conditions.     

Integrating wild mushrooms use into a model of sustainable management for indigenous community forests

Recently, the importance of fungal resources has been brought to the forefront due to their ecological and economical importance. There have been many efforts to record their diversity at local and regional scales. However, few studies deal with the potential value of fungi for the development of local communities, particularly in developing countries. The present study presents the results of a five-year inventory of useful mushrooms in the communal land of Ixtlan de Juarez, Oaxaca. We use the species list to analyze the real possibility of local communities incorporating these resources into a model of local development exploiting wood and non-wood forest products in the context of Smart wood certification. The model discusses three main strategies for mushroom management (diversification of forest exploitation, incorporation of soil function to forestry and fungal technology development); in each case we also discuss the viability and infrastructure necessary to implement it. We identified a total of 159 taxa of useful mushrooms and provide information on their habit, substrate, abundance and recorded use. Thirteen taxa were Ascomycetes and 146 Basidiomycetes; belonging to 42 families and 73 genera. A total of 38 taxa (23.9%) were new records for the state of Oaxaca. This is, to date, the most comprehensive list of useful mushrooms presented in Mexico. It demonstrates the huge mycological diversity of these resources present in the Juarez’ Mountain Range. A total of 113 species are edible, 38 species have direct applications in forest management, 19 species have medicinal properties, 12 are toxic and 12 species have recorded biotechnological applications. The diversity and heterogeneity of potential uses of these resources represent a challenging opportunity for local communities. Product diversification is a fundamental strategy to integrate a model of sustainable forest exploitation. We concluded that communities developing forestry activities must be aware of the functional properties of ectomycorrhizal and saprotrophic fungi to include them in their own forest management plans. We also outline a set of common steps for initiating sustainable and diversified forestry practices which include: awareness-raising targeted to local authorities and forestry technicians on the ecological importance of mushrooms; illustrated catalogues on useful species accessible to all the communities, which would allow taxonomic identification for all species; local technical capacity to obtain and disseminate strains of mycorrhizal and saprotrophic fungi; obtaining resources to develop infrastructure to cultivate mushrooms, among many others projects.     

Bartonellosis: an emerging infectious disease of zoonotic importance to animals and human beings

Objective – To provide a review of clinically relevant observations related to Bartonella species as emerging pathogens in veterinary and human medicine.
Data Sources – Literature as cited in PubMed and as generated by each of the authors who have contributed to various aspects of the clinical understanding of bartonellosis.
Human Data Synthesis – Important historical and recent publications illustrating the evolving role of animal reservoirs as a source of human infection.
Veterinary Data Synthesis – Comprehensive review of the veterinary literature.
Conclusions – In addition to inducing life-threatening illnesses, such as endocarditis, myocarditis, and meningoencephalitis and contributing to chronic debilitating disease, such as arthritis, osteomyelitis, and granulomatous inflammation in cats, dogs, and potentially other animal species; pets and wildlife species can serve as persistently infected reservoir hosts for the transmission of Bartonella spp. infection to veterinary professionals and others with direct animal contact.     

Polylepis australis’ regeneration niche in relation to seed dispersal,site characteristics and livestock density

Establishment of Polylepis forests endemic to the mountains of South America may be affected by seed dispersal, site characteristics and livestock density. Polylepis australis (“tabaquillo”) grows in the high mountains of central Argentina, where we set up 76 square study plots of 900 m². To determine dispersal distance, we distributed 6 seed traps per plot in and around 20 plots. To determine the best site characteristics and livestock stoking rates, at two river basins differing in historic stocking rates, we analyzed the presence of seedlings in 56 plots and recorded topography, vegetation types and indicators of livestock activity. We also measured microsite characteristics in a sample of 32 comparable pairs of 1 m² quadrats, with and without seedlings. Maximum recorded dispersal distance of P. australis seeds was 6 m, and seedlings were found no more than 10 m from seed trees. The numbers of seedlings and seed trees were 3.5 and 4 times higher, respectively, in the basin with less livestock. At the 900 m² plot scale, a Poisson regression indicated a positive relationship between seedling number and P. australis canopy cover. At the quadrat scale (1 m²), seedlings were found in quadrats with significantly lower evidence of soil erosion than comparison quadrats without seedlings. We conclude that the main limitations to recruitment are short seed dispersal distances, lack of seed trees and extreme soil erosion. Management should therefore aim at preserving seed trees and reducing livestock density to prevent erosion.